Recombinant Rabbit Monoclonal Antibody to Monomethylated Histone H3 Lysine 4, H3K4me1 (Clone: RM140)(Discontinued)

Product code: 10-9540

Clone name : RM140
Clonality : Monoclonal
Application : WB, ELISA, Multiplex, ChIP, ICC
Reactivity : All Species

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Format : Purified
Amount : 100 µg
Isotype : Rabbit IgG
Purification : Protein A affinity purified from an animal origin-free culture supernatant
Content : 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Storage condition : Store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : H3F3A
Gene ID : 3020
Uniprot ID : P84243
Alternative Name : Histone H3.3
Immunogen Information : A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4)

Clone RM140 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3 Western Blot: 0.2 µg/ml – 1 µg/ml; ChIP: 2 µg/ml- 10 µg/ml; ICC: 1 µg/ml - 2 µg/ml; ELISA: 0.2 µg/ml - 1 µg/ml; Multiplex: 0.1 µg/ml – 0.5 µg/ml.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus, Chromosome
Post transnational modification: Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.
BioGrid: 109273. 18 interactions.
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