Recombinant Rabbit Monoclonal Antibody to Histone H3 Unmodified Lysine 4 (Clone: RM186)(Discontinued)
Figure 1: Clone: RM186 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono- methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Rabbit IgG |
Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM186 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3. Western Blot: 1 µg/ml – 2 µg/ml; ELISA: 0.5 µg/ml - 1 µg/ml; Multiplex: 0.5 µg/ml – 2 µg/ml; Immunocytochemistry: 0.5 µg/ml- 2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Nucleus, Chromosome |
Post transnational modification: | Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis. |
Tissue Specificity: | Expressed in testicular cells. |
BioGrid: | 113895. 230 interactions. |
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