Recombinant Rabbit Monoclonal Antibody to Gamma H2AX (phospho-Ser139) (Clone: RM224)(Discontinued)
Figure 1: Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using Clone: RM224 at 0.5 µg/ml, showed a band of Histone H2A.X phosphorylated at serine 139 in HeLa cells.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. Western Blot: 0.5 µg/ml – 2 µg/ml; ELISA: 0.2 µg/ml - 1 µg/ml; Multiplex: 0.1 µg/ml – 1 µg/ml; ICC: 0.5 µg/ml - 2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome |
| Post transnational modification: | Acetylation at Lys-37 increases in S and G2 phases. This modification has been proposed to play a role in DNA double-strand break repair (By similarity). |
| BioGrid: | 109268. 310 interactions. |
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