Recombinant Rabbit Monoclonal Antibody to Dimethylated Histone H3 Lysine 79, H3K79me2 (Clone: RM181)(Discontinued)
Figure 1: Clone: RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM181 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). ). Very slightly cross-reacts with monomethylated Lysine 79 (K79me1) at high concentration. No cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3. Western Blot: 0.25 µg/ml – 1 µg/ml; IHC: 0.1 µg/ml – 1 µg/ml; ChIP: 2 µg/ml- 10 ug.ml; ELISA: 0.2 µg/ml - 1 µg/ml; Multiplex: 0.1 µg/ml – 0.5 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome |
| Post transnational modification: | Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis. |
| BioGrid: | 109273. 18 interactions. |
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