Recombinant Rabbit Monoclonal Antibody to CD43 (T-Cell Marker)(Clone : SPN/1766R)
Figure 1: Formalin-fixed, paraffin-embedded human Tonsil stained with CD43 Rabbit Recombinant Monoclonal Antibody (SPN/1766R).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Purified Ab with BSA and Azide at 200ug/ml |
| Content : | 200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Nucleus |
| Post transnational modification: | CD43 cytoplasmic tail: Sumoylated. |
| Tissue Specificity: | Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. |
| BioGrid: | 112571. 13 interactions. |
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