Recombinant Rabbit Monoclonal Antibody to Beta -actin (Clone: RM112)(Discontinued)
Figure 1: Immunocytochemical staining of HeLa cells, using anti- Beta -Actin Clone: RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM112 reacts to Alpha-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoform RMs of Actin. Western Blot (WB): 1:1000 dilution; Immunoprecipitation (IP): 1:200 dilution; Chromatin IP (ChIP): 1:200 dilution; Immunocytochemistry (ICC): 1:200 dilution; Immunohistochemistry (IHC): 1:200 dilution.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus |
| Post transnational modification: | (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
| BioGrid: | 106575. 336 interactions. |
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