Recombinant Rabbit Monoclonal Antibody to Alpha -Tubulin (Clone: RM113)(Discontinued)
Figure 1: Immunocytochemical staining of HeLa cells, using Anti- Alpha -Tubulin Clone: RM113 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM113 reacts to Alpha-Tubulin, including both Tubulin alpha-1A chain and Tubulin alpha-1B chain. Western Blot (WB): 1:1000 dilution; Immunoprecipitation (IP): 1:200 dilution; Chromatin IP (ChIP): 1:200 dilution; Immunocytochemistry (ICC): 1:200 dilution; Immunohistochemistry (IHC): 1:200 dilution.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Post transnational modification: | Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity). |
| BioGrid: | 115651. 161 interactions. |
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