Recombinant Rabbit Monoclonal Antibody to Alpha -Tubulin (Clone: RM113)(Discontinued)

Product code: 10-9536

Clone name : RM113
Clonality : Monoclonal
Application : IP, ELISA, IHC, FACS, WB

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Format : Purified
Amount : 100 µl
Isotype : Rabbit IgG
Purification : Protein A affinity purified from an animal origin-free culture supernatant
Content : 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Storage condition : Store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : TUBA1B
Gene ID : 10376
Uniprot ID : P68363
Alternative Name : TUBA1B
Immunogen Information : A peptide corresponding to the C-terminus of Alpha -Tubulin

Clone RM113 reacts to Alpha-Tubulin, including both Tubulin alpha-1A chain and Tubulin alpha-1B chain.  Western Blot (WB):  1:1000 dilution; Immunoprecipitation (IP): 1:200 dilution; Chromatin IP (ChIP):  1:200 dilution; Immunocytochemistry (ICC): 1:200 dilution; Immunohistochemistry (IHC): 1:200 dilution.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cytoplasm
Post transnational modification: Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).
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