Recombinant Rabbit Monoclonal Antibody to 5-methylcytosine (5-mC)(Clone: RM23)(Discontinued)
Figure 1: ELISA of single stranded DNA using rabbit monoclonal anti-5-mC (Clone: RM231) antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of Clone: RM231 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Format : | Purified |
Amount : | 50 µg |
Isotype : | Rabbit IgG |
Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Immunogen Information : | BSA-conjugated 5-methylcytosine. |
Clone RM231 reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. Dot Blot: 0.5 µg/ml – 2 µg/ml; ELISA: 0.1 µg/ml - 1 µg/ml; ICC: 0.5 µg/ml - 2 µg/ml; MeDIP: 0.2 µg/ml- 2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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