Recombinant Rabbit Monoclonal Antibody to 5-hydroxymethylcytosine (5-hmC); 5'-hmC (Clone: RM236)(Discontinued)
Figure 1: ELISA of single stranded DNA using rabbit monoclonal anti-5-hmC (Clone: RM236) antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of Clone: RM236 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Roll over image to zoom in
Shipping Info:
For estimated delivery dates, please contact us at [email protected]
| Format : | Purified |
| Amount : | 50 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
| Immunogen Information : | BSA-conjugated 5-hydroxymethylcytosine. |
Clone RM236 reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA. Dot Blot: 0.2 µg/ml – 1 µg/ml; ELISA: 0.1 µg/ml - 1 µg/ml; IHC: 0.1 µg/ml - 1 µg/ml; ICC: 0.5 µg/ml- 2 µg/ml; hMeDIP: 0.2 µg/ml- 2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|
There are currently no product reviews
|
.png "ACE2/HEK293 Stable Cell Line")
