Recombinant E. coli Formamidopyrimidine-DNA Glycosylase/Fpg (C-6His)(Discontinued)
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| Amount : | 50 µg |
| Content : | Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM EDTA, 1mM DTT, 50% Glycerol, pH 7.8. |
| Storage condition : | Store at -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. |
| AA sequence : | MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQKLEHHHHHH |
| Gene : | mutM |
| Uniprot ID : | A7ZTI6 |
Source: E.coli.
MW :31.4kD.
Recombinant E.coli Fpg is produced by our E.coli expression system and the target gene encoding Met1-Lys269 is expressed with a 6His tag at the C-terminus. Fpg is a DNA glycosylase that releases damaged bases preferentially from duplex DNA. It has an associated class I AP (apurinic/apyrimidinic) lyase activity.Fpg Cleaves the DNA backbone to generate a single-strand break at the site of the removed base, The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving 3' and 5' phosphoryl groups.
MW :31.4kD.
Recombinant E.coli Fpg is produced by our E.coli expression system and the target gene encoding Met1-Lys269 is expressed with a 6His tag at the C-terminus. Fpg is a DNA glycosylase that releases damaged bases preferentially from duplex DNA. It has an associated class I AP (apurinic/apyrimidinic) lyase activity.Fpg Cleaves the DNA backbone to generate a single-strand break at the site of the removed base, The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving 3' and 5' phosphoryl groups.
Endotoxin : Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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