Recombinant Anti-TNFa (Adalimumab biosimilar) mAb
Figure-1: SDS-Page analysis of Adalimumab. Adalimumab was purified by three step chromatography process followed by buffer exchange and concentration.
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| Amount : | 100 µg |
| Isotype : | Human IgG1, kappa |
| Purification : | Greater than 98.0% as determined by SDS-PAGE. |
| Content : | Adalimumab is supplied as solution in 60mM Trehalose, 0.7mM L-Histidine, 0.8mM L-His-Cl, and 30gm/lit Polysorbate 20. |
| Storage condition : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. |
SOURCE: Chinese Hamster Ovary Cells (CHO).
Adalimumab is a recombinant human IgG1 monoclonal antibody composed of two kappa light chains each with a molecular weight of approximately 24 kilo Daltons (kDa) and two IgG1 heavy chains each with a molecular weight of approximately 49 kDa based on the amino acid sequence. The total molecular weight of adalimumab with post-translational modifications is approximately 148 kDa. Each light chain consists of 214 amino acid residues and each heavy chain consists of 451 amino acid residues resulting in a total of 1330 amino acids for the entire IgG1 molecule; one glycosylation site (N301) is present. The primary amino-acid sequence of the heavy and light chains is reported.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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