rCarboxypeptidase-B Recombinant Protein
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| Amount : | 15 mg |
| Purification : | Greater than 90% as determined by SDS-PAGE. |
| Content : | The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5. |
| Storage condition : | Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Alternative Name : | Carboxypeptidase B, Cpb1, Cpb. |
Source : Escherichia Coli.
Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 35kDa is purified by standard chromatography techniques.
Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.
Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kda, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.
It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 170 units/mg protein.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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