Rat Granulocyte Macrophage-Colony Stimulating Factor
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| Amount : | 100 µg |
| Purification : | Reducing and Non-Reducing SDS PAGE at >= 95% |
| Content : | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium bicarbonate, pH 8.5 Sterile water at 0.1 mg/mL |
| Storage condition : | Store at -20°C |
| AA sequence : | MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK |
Monomer, 14.7 kDa (128 aa)
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is hematopoietic factor produced by entothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity.Â
Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Biological Activity was determined by FDC-P1 cell proliferation at <=20 pg/mL; >= 5.0 x 107 units/mg. Centrifuge vial before opening, Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.  Â
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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