Rat Fibroblast Growth Factor-basic (AF)

Product code: 32-12071

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  •   50 µg

  •  100 µg

  • $430.00 

  • $600.00 

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Amount : 100 µg
Purification : Reducing and Non-Reducing SDS PAGE at >= 95%
Content : Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Sterile water at 0.1 mg/mL
Storage condition : Store at -20°C
AA sequence : MPALPEDGGG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Gene : Fgf2
Gene ID : 54250
Uniprot ID : P13109
Alternative Name : Fgf-2, Basic fibroblast growth factor, Heparin-binding growth factor 2
Source: Genetically modified E.coli.
Predicted MW: Monomer, 16.4 kDa (146 aa)
Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic binds heparin in order to signal through fibroblast growth factor (FGF) receptor tyrosine kinases.

Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Biological Activity was determined by 3T3 Proliferation. at <=1 ng/mL; >= 1.0 x 10^6 units/mg (typical ED50 is < 100 pg/mL). Centrifuge vial before opening, Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Secreted, Nucleus
Post transnational modification: Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion.
Tissue Specificity: Found in all tissues examined.
BioGrid: 248473. 1 interactions.
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