Rabbit Polyclonal Antibody to Histone H3 (Discontinued)
Figure-1 : Western blot analysis of Histone H3 Antibody at 1 µg/ml on mouse brain, Rat brain tissue lysates & Hela, NIH3T3, A549, K562, Jurkat, SH-SY-5Y, LOVO cell lysates, IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Rabbit IgG |
| Purification : | Immunoaffinity chromatography. |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Histone H3 is the core component of nucleosome. It has been implicated in nucleosomes wrap and compacts DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Rabbit Anti-Histone H3 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human Histone H3 (Swiss Prot: P68431).
Western blot: 0.5-1 µg/ml
ICC/IF: 1-3 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome |
| Post transnational modification: | Serine ADP-ribosylation constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:30257210). Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac) (PubMed:30257210). |
| BioGrid: | 114458. 10 interactions. |
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