Rabbit Polyclonal Antibody to DDDDK-tag (Discontinued)
Figure-1 : Western blot analysis of DDDDK-tag Antibody on DDDDK tagged fusion protein in Lane 1-3 ( 10, 1 & 0.1 ng) expressed in E. coli lysates, Lane- 4: E. coli lysates as Negative Control.
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Rabbit IgG |
| Purification : | Immunoaffinity chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Immunogen Information : | A synthetic epitope peptide xxxDDDDK conjugated to KLH. This sequence represents the enterokinase cleavage site . |
Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems. The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins. The Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
ELISA: 0.05-0.2 µg/ml
Western blot:1-2 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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