Rabbit Polyclonal Antibody to Avi-tag
Figure-1 : Western blot analysis of Avi-tag Antibody at 1 μg/ml on Avi-tagged fusion protein (1000, 200, 40 & 8 ng)expressed in E. coli cell lysate.
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Rabbit IgG |
| Purification : | Immunoaffinity chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Immunogen Information : | Avi tag peptide conjugated to KLH |
Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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