Quick-blots (Rat Tissues)
Fig-1: Quick-blotsTM of rat tissue (Cat No.: 16-1002): Different Rat tissue Lysates around 20 μg per lane were resolved by SDS-PAGE, trasfered on to PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker, 2. Brain, 3. Heart, 4. Small Intestine, 5. Kidney, 6. Liver, 7. Lung, 8. Skeletal Muscle, 9. Stomach, 10. Spleen, 11. Ovary, 12. Testis.
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Shipping Info:
Order now and get it on Friday November 29, 2024
Same day delivery FREE on San Diego area orders placed by 1.00 PM
| Amount : | 1 Membrane |
| Content : | 1 pre-transferred membrane |
| Storage condition : | Store unopened at RT. For long-term, store at 4â°C. |
Quick-blotsTM is Ready-to-use PVDF membrane with 11 Rat tissue lysates (20 µg total protein per lane). Quick-blotsTM is a very simple method to identify different proteins from rat tissues.
Protocol:
1. Activate the membrane with Methanol for 1-2 min and then wash the blots with TBST (25 mM Tris-Cl, pH 8.0; 125 mM NaCl; 0.1% Tween 20) buffer thrice to get rid of extra methanol.
2. Block the membrane for 1 hour in 5% skimmed milk ( in TBST) to avoid non specific binding of antibodies.
3. Wash the membranes 2 times in TBST and incubate with primary antibodies prepared in 1% skimmed milk for 2 hr in RT/ over night at 4oC.
4. After primary incubation, wash the membranes for 1 hour in TBST at RT. (Buffer should be changed in 30 min interval)
5. Incubate the membranes in appopriate Secondry antibody for 1 hour.
6. After secondary incubation, wash the membranes for 2 hours (Buffer should be changed in 30 min interval). Then develop the blots with Chemiluminescent Substrate and interpret the results in photographic film.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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