Quick-blot (Mouse Tissues)
Fig 1: Quick-blotsTM Mouse Tissues (Cat No.: 16-1001): Different Mouse tissue Lysates around 20 μg per lane were resolved by SDS-PAGE, trasfered on to PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker, 2. Brain, 3. Heart, 4. Small Intestine, 5. Kidney, 6. Liver, 7. Lung, 8. Muscle, 9. Stomach, 10. Spleen, 11. Ovary, 12. Testis
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Shipping Info:
For estimated delivery dates, please contact us at [email protected]
| Amount : | 1 Membrane |
| Content : | 1 pre-transferred membrane |
| Storage condition : | Store unopened at RT. For long-term, store at 4â°C. |
Quick-blotsTM is Ready-to-use PVDF membrane with 11 mouse tissue lysates (20 μg total protein per lane). Quick-blotsTM is a very simple method to identify different mouse proteins.
Quick-blotsTM Protocol:
- Activate the membrane with Methanol for 1-2 min and then wash the blots with TBST (25 mM Tris-Cl, pH 8.0; 125 mM NaCl; 0.1% Tween 20) buffer thrice to get rid of extra methanol.
- Block the membrane for 1 hour in 5% skimmed milk ( in TBST) to avoid non specific binding of antibodies.
- Wash the membranes 2 times in TBST and incubate with primary antibodies prepared in 1% skimmed milk for 2 hr in RT/ over night at 4â°C.
- After primary incubation, wash the membranes for 1 hour in TBST at RT. (Buffer should be changed in 30 min interval)
- Incubate the membranes in appopriate Secondry antibody for 1 hour.
- After secondary incubation, wash the membranes for 2 hours (Buffer should be changed in 30 min interval). Then develop the blots with Chemiluminescent Substrate and interpret the results in photographic film .
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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