Polyclonal Antibody to Zap-70 (Ab-319)

Figure 1: Western blot analysis of extracts from HL60 cells using Zap-70(Ab-319) Antibody 35-1515 .

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Figure 1: Western blot analysis of extracts from HL60 cells using Zap-70(Ab-319) Antibody 35-1515 .

Figure 2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70(Ab-319) Antibody 35-1515 (left) or the same antibody preincubated with blocking peptide(right).

Product code: 35-1515

Clonality :	Polyclonal
Application :	WB, IHC
Reactivity :	Human, Mouse, Rat

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit IgG
Content :	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	ZAP70
Gene ID :	7535
Uniprot ID :	P43403
Alternative Name :	70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70
Immunogen Information :	Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448 Ku GM, et al. (2001) EMBO J; 20(3): 457-465 Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780 Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774 Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844 Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.

Predicted MW: 70kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cell membrane
Post transnational modification:	Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
Tissue Specificity:	Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
BioGrid:	113367. 52 interactions.