Polyclonal Antibody to Vimentin

Name: Polyclonal Antibody to Vimentin
Brand: Abeomics
SKU: 34-1126
Price: 462.0000 USD
Availability: InStock
Rating: 4.9 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cell culture stained with chicken pAb to vimentin,(34-1126), dilution 1:10,000 in green, and costained with mouse mAb to actin,(34-1002), dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

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Figure-1: Immunofluorescent analysis of HeLa cell culture stained with chicken pAb to vimentin,(34-1126), dilution 1:10,000 in green, and costained with mouse mAb to actin,(34-1002), dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

Figure-2: Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, (34-1126), dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. (34-1126) binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes while the ~70kDa bands are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.

Product code: 34-1126

Clonality :	Polyclonal
Application :	WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Conc. IgY prep.
Amount :	100 µl
Isotype :	Chicken, IgY
Content :	Antibody is supplied as an aliquot of concentrated IgY prep.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	VIM
Gene ID :	7431
Uniprot ID :	P08670
Immunogen Information :	Recombinant human vimentin expressed in and purified from E. coli.

WB: 1:5,000. IF/ICC and IHC: 1:10,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cytoplasm, Nucleus matrix
Post transnational modification:	S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
BioGrid:	113272. 291 interactions.