Polyclonal Antibody to Vimentin

Name: Polyclonal Antibody to Vimentin
Brand: Abeomics
SKU: 34-1129
Price: 462.0000 USD
Availability: InStock
Rating: 4.1 (10 reviews)

Figure-1: Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin,(34-1129), dilution 1:5,000, in green, and mouse mAb to Actin,(34-1002), dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

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Figure-1: Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin,(34-1129), dilution 1:5,000, in green, and mouse mAb to Actin,(34-1002), dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

Figure-2: Western blot analysis of whole cell lysates using rabbit pAb to vimentin,(34-1129), dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa.

Product code: 34-1129

Clonality :	Polyclonal
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Sera
Amount :	100 µl
Isotype :	Rabbit, IgG
Content :	Antibody is supplied as an aliquot of serum
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	VIM
Gene ID :	7431
Uniprot ID :	P08670
Immunogen Information :	Recombinant human vimentin expressed in and purified from E. coli

Western blot: 1:10,000. IF/ICC and IHC: 1:5,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cytoplasm, Nucleus matrix
Post transnational modification:	S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
BioGrid:	113272. 291 interactions.