Polyclonal Antibody to Vimentin
Figure-1: Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin,(34-1129), dilution 1:5,000, in green, and mouse mAb to Actin,(34-1002), dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
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Shipping Info:
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| Format : | Sera |
| Amount : | 100 µl |
| Isotype : | Rabbit, IgG |
| Content : | Antibody is supplied as an aliquot of serum |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Western blot: 1:10,000. IF/ICC and IHC: 1:5,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cytoplasm, Nucleus matrix |
| Post transnational modification: | S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| BioGrid: | 113272. 291 interactions. |
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