Polyclonal Antibody to Ubiquitin C-terminal hydrolase 1, UCHL1

Name: Polyclonal Antibody to Ubiquitin C-terminal hydrolase 1, UCHL1
Brand: Abeomics
SKU: 34-1122
Price: 462.0000 USD
Availability: InStock
Rating: 4.3 (10 reviews)

Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1,(34-1122) , dilution 1:500 in red, and costained with mouse mAb to vimentin, (34-1127), dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.

Polyclonal Antibody to Ubiquitin C-terminal hydrolase 1, UCHL1

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Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1,(34-1122) , dilution 1:500 in red, and costained with mouse mAb to vimentin, (34-1127), dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.

Figure-2: Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1,(34-1122), dilution 1:2,000 in green, and mouse mAb to Actin,(34-1002), dilution 1:1,000, in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.

Product code: 34-1122

Clonality :	Polyclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human, Rat, Mouse, Cow, Pig, Horse

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Conc. IgY prep.
Amount :	100 µl
Isotype :	Chicken, IgY
Content :	Antibody is supplied as an aliquot of concentrated IgY prep.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	UCHL1
Gene ID :	7345
Uniprot ID :	P09936
Alternative Name :	Neuron cytoplasmic protein 9.5, PGP9.5, PGP 9.5, Ubiquitin thioesterase L1
Immunogen Information :	Recombinant full length human UCHL1 purified from E. coli.

WB: 1:2,000-5,000. IF/ICC and IHC: 1:500-1,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Endoplasmic reticulum membrane
Post transnational modification:	O-glycosylated.
Tissue Specificity:	Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
BioGrid:	113192. 58 interactions.