Polyclonal antibody to Survivin

Fig:1 Western blot analysis of Survivin using 20-1090 at 1:2000. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombiant human survivin protein was used as a positive control.

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Fig:1 Western blot analysis of Survivin using 20-1090 at 1:2000. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombiant human survivin protein was used as a positive control.

Fig:2 Immunohistochemical analysis of Survivin in formalin-fixed, paraffin-embedded human prostate and breast cancer using 20-1090 at 1:2000. A and A1, prostate cancer at low and high magnification, respectively. B and B1, breast cancer. B1 is a high magnification of an area of metastasis. Hematoxylin-eosin counterstain.

Fig:3 Immunohistochemical analysis of Survivin in a formalin-fixed, paraffin-embedded human brain tissue microarray using 20-1090 at 1:2000. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.

Product code: 20-1090

Clonality :	Polyclonal
Application :	IP, IHC, WB
Reactivity :	Rat, Mouse, Human

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Download TDS MSDS

Format :	Sera
Amount :	50 µl
Isotype :	Rabbit IgG
Content :	50 µl sera
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	BIRC5
Gene ID :	332
Uniprot ID :	O15392
Alternative Name :	Baculoviral IAP repeat-containing protein 5,Apoptosis inhibitor 4, Apoptosis inhibitor survivin
Immunogen Information :	A Full-length recombinant protein of human Survivin was used as immunogen for this antibody

Survivin is a member of the family of inhibitor of apoptosis proteins (IAP), and is involved in both the control of apoptosis and regulation of cell division. IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains.It is a 142 amino acid protein with an approximate molecular weight of 16.5 kDa. Structurally, survivin is composed of a single BIR domain and an extended COOH-terminal a-helical coiled-coil domain and is highly expressed in embryonic and fetal organs.Survivin is undetectable in most normal differentiated and non-proliferating tissues, but is highly expressed in majority of human tumor types including lung, breast, colon, gastric, leukemias, neuroblastoma, brain tumors, pheochromocytoma, soft tissue sarcomas, melanomas and other skin tumors. Additionally, survivin expression has also been detected in a variety of preneoplastic or benign leisons, suggesting that re-expression of survivin may occur early during malignant transformation or following a disruption in the balance between cell division and apoptosis.

WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Nucleus, Chromosome, Chromosome, Cytoplasm
Post transnational modification:	Ubiquitination is required for centrosome-targeting.
BioGrid:	98984. 5 interactions.