Polyclonal Antibody to STAT1 (Ab-701)
Figure 1: Western blot analysis of extracts from Hela, A431 and JK cells using STAT1(Ab-701) Antibody 35-1399 .
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Shipping Info:
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Durbin J E, et al. (1996) Cell. 84: 443-450. Meraz M A, et al. (1996) Cell. 84: 431-442. Wakao H, et al. (1994) EMBO J. 13: 2182-2191. Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258 Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.
Predicted MW: 84,91kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus |
| Post transnational modification: | Monomethylated at Lys-525 by SETD2; monomethylation is necessary for phosphorylation at Tyr-701, translocation into the nucleus and activation of the antiviral defense. |
| BioGrid: | 112649. 171 interactions. |
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