Polyclonal Antibody to PKM2(phospho-Ser37)
Figure 1: Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody 35-1300 .
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Weiwei Yang1, Yanhua Zheng1, Yan Xia1, Haitao Ji, "ERK1/2-dependent phosphorylation and nucleartranslocation of PKM2 promotes the Warburg effect." Nature Cell Biology(2012)|doi:10.1038/ncb2629
Received16 August 2012 Accepted24 October 2012 Published online25 November 2012
Predicted MW: 60kd, Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus |
| Post transnational modification: | FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. |
| Tissue Specificity: | Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. |
| BioGrid: | 111332. 217 interactions. |
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