Polyclonal Antibody to PDK1 (Phospho-Ser241)
Figure 1: Western blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1(Phospho-Ser241) Antibody 35-1004 .
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.
Predicted MW: 63kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane, Cell junction |
| Post transnational modification: | Monoubiquitinated in the kinase domain, deubiquitinated by USP4. |
| Tissue Specificity: | Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast. |
| BioGrid: | 111196. 61 interactions. |
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