Polyclonal antibody to NOD2 (Card15)
Fig:1 Western blot analysis of NOD2 using 20-1115 at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.
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Format : | Sera |
Amount : | 50 µl |
Isotype : | Rabbit IgG |
Content : | 50 µl sera |
Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm, Membrane, Mitochondrion, Basolateral cell membrane |
Post transnational modification: | Polyubiquitinated following MDP stimulation, leading to proteasome-mediated degradation (PubMed:23019338). |
Tissue Specificity: | Expressed in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium. |
BioGrid: | 122077. 34 interactions. |
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