Polyclonal Antibody to Neurofilament NF-M
Figure-1: Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M,(34-1087), dilution 1:1,000 in red, and costained with mouse mAb to CNP, dilution 1:500 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The NF-M antibody labels the network of axons of basket neurons and other neurons. The CNP antibody stains oligodendrocytes, cells that create myelin sheaths around axons.
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Shipping Info:
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Format : | Conc. IgY prep. |
Amount : | 100 µl |
Isotype : | Chicken, IgY |
Content : | Antibody is supplied as an aliquot of concentrated IgY prep in PBS with 0.02% NaN3 (total concentration is 26mg/ml) |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:2,000-5,000, IF/ICC & IHC: 1:500-1,000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Post transnational modification: | Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
BioGrid: | 110818. 48 interactions. |
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