Polyclonal Antibody to Neurofilament NF-H

Name: Polyclonal Antibody to Neurofilament NF-H
Brand: Abeomics
SKU: 34-1080
Price: 500.0000 USD
Availability: InStock
Rating: 4.5 (10 reviews)

Figure-1: Immunohistological analysis of a rat cerebellum section stained with chicken pAb to NF-H, (34-1080), dilution 1:5,000 in red, and costained with rabbit pAb to GFAP,(34-1042), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

Polyclonal Antibody to Neurofilament NF-H

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Figure-1: Immunohistological analysis of a rat cerebellum section stained with chicken pAb to NF-H, (34-1080), dilution 1:5,000 in red, and costained with rabbit pAb to GFAP,(34-1042), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

Figure-2: Western blot analysis of spinal cord lysates from different species using chicken pAb to NF-H, (34-1080), dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H (not shown, but see reference 1).

Product code: 34-1080

Clonality :	Polyclonal
Application :	WB, IF/ICC, IHC, ELISA
Reactivity :	Human, Rat, Mouse, Cow, Pig, Dog, Horse

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Conc. IgY prep.
Amount :	50 µl
Isotype :	Chicken, IgY
Content :	Antibody is supplied as an aliquot of concentrated IgY prep.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	NEFH
Gene ID :	4744
Uniprot ID :	P12036
Alternative Name :	200 kDa neurofilament protein, Neurofilament triplet H protein
Immunogen Information :	Native NF-H purified from bovine spinal cord.

WB: 1:20,000-1:50,000. IF/ICC, IHC: 1:20,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm
Post transnational modification:	Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
BioGrid:	110819. 25 interactions.