Polyclonal Antibody to MEK1 (Phospho-Ser221)
Figure 1: Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody 35-1154 .
Roll over image to zoom in
Shipping Info:
For estimated delivery dates, please contact us at [email protected]
| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8.
Luciano BS, et al. (2006)J Biol Chem; 279(50): 52117-23.
Wang X, et al. (2003) Oncogene; 22(1): 109-16.
Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25.
Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.
Predicted MW: 45kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cytoplasm, Cytoplasm, Nucleus, Membrane |
| Post transnational modification: | Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Tissue Specificity: | Widely expressed, with extremely low levels in brain. |
| BioGrid: | 111590. 121 interactions. |
|
There are currently no product reviews
|
.png "ACE2/HEK293 Stable Cell Line")
