Polyclonal Antibody to MARCKS

Name: Polyclonal Antibody to MARCKS
Brand: Abeomics
SKU: 34-1062
Price: 462.0000 USD
Availability: InStock
Rating: 4.8 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cells stained with rabbit pAb to MARCKS,(34-1062), dilution 1:1,000 in red, and costained with mouse mAb to Î²-tubulin,(34-1116), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The (34-1062) antibody recognizes protein localized in the plasma membrane and cytoplasm, while the Î²-tubulin antibody stains the network of cytoplasmic microtubules.

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Figure-1: Immunofluorescent analysis of HeLa cells stained with rabbit pAb to MARCKS,(34-1062), dilution 1:1,000 in red, and costained with mouse mAb to Î²-tubulin,(34-1116), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The (34-1062) antibody recognizes protein localized in the plasma membrane and cytoplasm, while the Î²-tubulin antibody stains the network of cytoplasmic microtubules.

Figure-2: Western blot analysis of cell line lysates probed simultaneously with rabbit pAb to MARCKS,(34-1062), dilution 1:1,000, in green and mouse mAb to GAPDH, (34-1047), dilution 1:5,000 in red: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6 cells. The strong band at ~80kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells [5], but no reactivity is seen on rodent cells, [6] and [7]. GAPDH antibody used as a loading control revealing a single band at ~37kDa in all preparations.

Product code: 34-1062

Clonality :	Polyclonal
Application :	WB, IF/ICC
Reactivity :	Human, not rodent

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Sera
Amount :	100 µl
Isotype :	Rabbit, IgG
Content :	Antibody is supplied as an aliquot of serum
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	MARCKS
Gene ID :	65108
Uniprot ID :	P49006
Alternative Name :	MARCKS-like protein 1, Mac-MARCKS, Macrophage myristoylated alanine-rich C kinase substrate
Immunogen Information :	Full length recombinant human MARCKS purified from E. coli

WB: 1:10,000-1:20,000. IF/ICC and IHC: 1:500-1:1,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cell membrane
Post transnational modification:	Phosphorylated. Phosphorylation at Ser-120 and Thr-178 is non-redundantly catalyzed by MAPK8 in vivo. Phosphorylation at Thr-148 is preferentially catalyzed by MAPK8 in vivo, but this modification can also be catalyzed by other kinases in the absence of MAPK8. May be phosphorylated by protein kinase C, which disrupts the interaction with calmodulin.
BioGrid:	122395. 45 interactions.