Polyclonal Antibody to LC3
Figure 1: Western blot analysis of extract from K-562, Hela, JK and 293 cells using LC3 Antibody 35-1656
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Predicted MW: 19kd, Western blotting: 1:500~1:1000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Endomembrane system, Cytoplasmic vesicle, Cytoplasmic vesicle |
| Post transnational modification: | Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity. |
| Tissue Specificity: | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. |
| BioGrid: | 124137. 121 interactions. |
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