Polyclonal Antibody to Lamin A/C

Name: Polyclonal Antibody to Lamin A/C
Brand: Abeomics
SKU: 34-1057
Price: 462.0000 USD
Availability: InStock
Rating: 4.5 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C,(34-1057), dilution 1:2,000 in red, and costained with mouse mAb to actin,(34-1002), dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The (34-1057) antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

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Figure-1: Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C,(34-1057), dilution 1:2,000 in red, and costained with mouse mAb to actin,(34-1002), dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The (34-1057) antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

$Figure-2: Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C,(34-1057), dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65kDa and 74kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, (34-1047), in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.$

Product code: 34-1057

Clonality :	Polyclonal
Application :	WB, IF/ICC
Reactivity :	Human, Rat, Mouse, Horse, Monkey, Dog

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Conc. IgY prep.
Amount :	100 µl
Isotype :	Chicken, IgY
Content :	Antibody is supplied as an aliquot of IgY preparation.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	LMNA
Gene ID :	4000
Uniprot ID :	P02545
Alternative Name :	70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMN1
Immunogen Information :	Full length human Lamin A purified from E. coli.

WB: 1:2,000. IF/ICC 1:1,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus speckle
Post transnational modification:	Farnesylation of prelamin-A/C facilitates nuclear envelope targeting.
Tissue Specificity:	In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.
BioGrid:	110186. 661 interactions.