Polyclonal Antibody to Keratin 8 (Phospho-Ser74)
Figure 1: Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Keratin 8(Phospho-Ser74) Antibody 35-1277 .
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751
Predicted MW: 55kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Nucleus matrix |
| Post transnational modification: | O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
| Tissue Specificity: | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. |
| BioGrid: | 110054. 69 interactions. |
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