Polyclonal Antibody to Histone H3.1 (Phospho-Ser10)

Figure 1: Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody 35-1174 .

Polyclonal Antibody to Histone H3.1 (Phospho-Ser10)

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Figure 1: Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody 35-1174 .

Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1(Phospho-Ser10) Antibody 35-1174 (left) or the same antibody preincubated with blocking peptide(right).

Figure 3: Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Histone H3.1 (Phospho-Ser10) Antibody 35-1174 .

Figure 4: Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody 35-1174 .

Product code: 35-1174

Clonality :	Polyclonal
Application :	WB, IHC, IF
Reactivity :	Human, Mouse, Rat

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit IgG
Content :	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	HIST1H3A
Gene ID :	8350
Uniprot ID :	P68431
Alternative Name :	H3/b, H3FB
Immunogen Information :	Peptide sequence around phosphorylation site of serine 10 (R-K-S(p)-T-G) derived from Human Histone H3.1.

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Dai J, et al. (2005) Genes Dev 19(4): 472-488. Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.

Predicted MW: 17kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus, Chromosome
Post transnational modification:	Serine ADP-ribosylation constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:30257210). Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac) (PubMed:30257210).
BioGrid:	114458. 10 interactions.