Polyclonal Antibody to Elk-1 (Phospho-Ser383)

Figure 1: Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1(Phospho-Ser383) Antibody 35-1003 .

Polyclonal Antibody to Elk-1 (Phospho-Ser383)

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Figure 1: Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1(Phospho-Ser383) Antibody 35-1003 .

Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1(Phospho-Ser383) Antibody 35-1003 (left) or the same antibody preincubated with blocking peptide(right).

Product code: 35-1003

Clonality :	Polyclonal
Application :	WB, IHC
Reactivity :	Human, Mouse, Rat

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit IgG
Content :	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	ELK1
Gene ID :	2002
Uniprot ID :	P19419
Alternative Name :	ELK1, ETS-domain protein Elk-1
Immunogen Information :	Peptide sequence around phosphorylation site of serine 383 (T-L-S(p)-P-I) derived from Human Elk-1.

Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.

Predicted MW: 47kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus
Post transnational modification:	On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).
Tissue Specificity:	Lung and testis.
BioGrid:	108317. 28 interactions.