Polyclonal Antibody to CREB (Phospho-Ser133)

Figure 1: Western blot analysis of extracts from Hela cells untreated or treated with UV using CREB(Phospho-Ser133) Antibody 35-1051 .

Polyclonal Antibody to CREB (Phospho-Ser133)

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Figure 1: Western blot analysis of extracts from Hela cells untreated or treated with UV using CREB(Phospho-Ser133) Antibody 35-1051 .

Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser133) Antibody 35-1051 (left) or the same antibody preincubated with blocking peptide(right).

Product code: 35-1051

Clonality :	Polyclonal
Application :	WB, IHC
Reactivity :	Human, Mouse, Rat

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit IgG
Content :	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	CREB1
Gene ID :	1385
Uniprot ID :	P16220
Alternative Name :	CREB-1, CREB1
Immunogen Information :	Peptide sequence around phosphorylation site of serine 133 (R-P-S(p)-Y-R) derived from Human CREB.

CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories . Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55. Tan Y, et al. (1996) EMBO J; 15(17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.

Predicted MW: 43kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus
Post transnational modification:	Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
BioGrid:	107775. 146 interactions.