Polyclonal Antibody to CLIM1/PDLIM1
Fig-1: Western blot analysis of CLIM1. Anti- CLIM1 antibody (11-8007) was used at 1 µg/ml on 1) Heart, 2) SKM and 3) HepG2 lysates.
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Rabbit IgG |
Purification : | Protein A Chromatography |
Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
PDLIM1 (PDZ and LIM Domain 1) belongs to the enigma protein family. It contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxy terminal. LIM domains are cysteine-rich double zinc fingers that are involved in protein-protein interactions. PDLIM1 has transcription coactivatior activity and it may play a role as an adapter to bring other LIM-interacting proteins to the cytoskeleton. This gene is found to be highly expressed in the heart and skeletal muscle and moderately expressed in the spleen. PDLIM1 consists of 329 amino acids and is associated with hyperacusis and supravalvular aortic stenosis diseases.
Western blot analysis: 1-3 µg/ml, Immunohistochemical analysis: 5 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm, Cytoplasm, Cytoplasm |
Tissue Specificity: | Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes. |
BioGrid: | 114572. 35 interactions. |
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