Polyclonal Antibody to Chk1 (Ab-317)
Figure 1: Western blot analysis of extracts from MDA231, Hela and 293 cells using Chk1 (Ab-317) Antibody 35-1465 .
Roll over image to zoom in
Shipping Info:
For estimated delivery dates, please contact us at [email protected]
| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry
Zhang YW, et al. (2005) Mol Cell; 19(5): 607-18.
Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87.
Rocha S, et al. (2005) EMBO J.
Clarke CA, et al. (2005) Biochem J.
Yu X, et al. (2004) Mol Cell Biol; 24(21): 9478-86.
Predicted MW: 56kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Cytoplasm, Cytoplasm |
| Post transnational modification: | Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity). The activated form (phosphorylated on Ser-345) is polyubiquitinated at Lys-436 by some SCF-type E3 ubiquitin ligase complex containing FBXO6 promoting its degradation. Ubiquitination and degradation are required to terminate the checkpoint and ensure that activated CHEK1 does not accumulate as cells progress through S phase, when replication forks encounter transient impediments during normal DNA replication. |
| Tissue Specificity: | Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. |
| BioGrid: | 107536. 159 interactions. |
|
There are currently no product reviews
|
.png "ACE2/HEK293 Stable Cell Line")
