Polyclonal antibody to Caspase-6
Fig:1 Western blot analysis of Caspase-6 using Caspase-6 (20-1041) polyclonal antibody. Recombinant catalytically active caspase-6 was Fig:1 Western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.
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Format : | Sera |
Amount : | 50 µl |
Isotype : | Rabbit IgG |
Content : | 50 µl sera |
Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Caspases belong to the cysteine-aspartic acid protease (Caspase) family which plays a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis-inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase-6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.This Active/Cleaved Caspase-6 polyclonal antibody recognizes the large and small subunits of caspase-6. Whereas the antisera has a strong preference for active/cleaved caspase-6, in some cell or tissue systems or techniques the antisera may also recognize the ~34 kDa proform of caspase-6 as well as intermediate caspase-6 cleavage fragments.
WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm |
Post transnational modification: | Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. |
BioGrid: | 107289. 19 interactions. |
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