Polyclonal antibody to CARD8 (Tucan)
Fig:1 Western blot analysis of TUCAN in five matched pairs of normal colonic mucosa (N) and colon carcinoma (T) using 20-1105 at 1:2000. Specimens were normalized for total protein content. Each set of N and T is tissue lysate from the same patient.
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| Format : | Sera |
| Amount : | 50 µl |
| Isotype : | Rabbit IgG |
| Content : | 50 µl sera |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
This antibody recognizes TUCAN, a 431 amino acid protein. TUCAN (tumor up-regulated CARD-containing antagonist of caspase nine) also known as CARD8 is a CARD domain containing protein. Proteins containing a CARD (caspase-associated recruitment domain) domain are key regulators of cell death, cell survival and cytokine production. TUCAN is an anti-apoptotic CARD protein that can protect tumors from cell death stimuli, and is overexpressed in certain forms of cancer. TUCAN has been shown to inhibit caspase-9 activation by binding to the CARD region of pro-caspase-9, thereby suppressing the formation of the Apaf-1-caspase-9 apoptotic complex and apoptosis. Additionally, a TUCAN isoform has been described that blocks both caspase-8 and caspase-9 mediated apoptosis. However, in some tumors, TUCAN play a role in modulating NFkB transcription factor survivial signaling pathways.
WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus |
| Tissue Specificity: | High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow. |
| BioGrid: | 116564. 45 interactions. |
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