Polyclonal Antibody to Calbindin

Figure-1: Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to calbindin,(34-1020), dilution 1:2,000, in green, and costained with rabbit pAb to MeCP2,(34-1063), dilution 1:5,000, in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.