Polyclonal Antibody to c-Fos(Discontinued)

Figure 1: Left: 34-1033 staining (red) in HeLa cells which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or followed by PBS treatment (top panel). Red c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells were counterstained with our chicken polyclonal antibody against vimentin, 34-1126 (green). Blue shows DAPI staining of nuclear DNA?Middle: Mouse brain section (45 ?M; fixed by transcardial perfusion with 4% paraformaldehyde) labeled with c-Fox-AP using a standard HRP-DAB staining technique. Cells expressing c-Fos appear to be dark. Right: Mouse brain section across hippocampus labeled with 34-1033 (red) and our anti Fox3/NeuN ( 34-1035 ) antibody (green) using immuno-fluorescent microscopy. Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of 34-1033 staining. Nuclei are labeled with Dapi (blue). For more images of stimulated and non-stimulated cells and tissues stained with 34-1033 and our monoclonal antibody to c-Fos, 34-1032 press here.