Polyclonal Antibody to c-Fos(Discontinued)

Name: Polyclonal Antibody to c-Fos(Discontinued)
Brand: Abeomics
SKU: 34-1033
Price: 280.0000 USD
Availability: InStock
Rating: 4.3 (10 reviews)

Figure 1: Left: 34-1033 staining (red) in HeLa cells which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or followed by PBS treatment (top panel). Red c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells were counterstained with our chicken polyclonal antibody against vimentin, 34-1126 (green). Blue shows DAPI staining of nuclear DNA?Middle: Mouse brain section (45 ?M; fixed by transcardial perfusion with 4% paraformaldehyde) labeled with c-Fox-AP using a standard HRP-DAB staining technique. Cells expressing c-Fos appear to be dark. Right: Mouse brain section across hippocampus labeled with 34-1033 (red) and our anti Fox3/NeuN ( 34-1035 ) antibody (green) using immuno-fluorescent microscopy. Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of 34-1033 staining. Nuclei are labeled with Dapi (blue). For more images of stimulated and non-stimulated cells and tissues stained with 34-1033 and our monoclonal antibody to c-Fos, 34-1032 press here.

Polyclonal Antibody to c-Fos(Discontinued)

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Figure 1: Left: 34-1033 staining (red) in HeLa cells which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or followed by PBS treatment (top panel). Red c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells were counterstained with our chicken polyclonal antibody against vimentin, 34-1126 (green). Blue shows DAPI staining of nuclear DNA?Middle: Mouse brain section (45 ?M; fixed by transcardial perfusion with 4% paraformaldehyde) labeled with c-Fox-AP using a standard HRP-DAB staining technique. Cells expressing c-Fos appear to be dark. Right: Mouse brain section across hippocampus labeled with 34-1033 (red) and our anti Fox3/NeuN ( 34-1035 ) antibody (green) using immuno-fluorescent microscopy. Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of 34-1033 staining. Nuclei are labeled with Dapi (blue). For more images of stimulated and non-stimulated cells and tissues stained with 34-1033 and our monoclonal antibody to c-Fos, 34-1032 press here.

Figure 2: Top panel : Western blot analysis of c-Fos expression HeLa cells with 34-1033 . 1: HeLa cells were serum-starved for 36 hours?2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours?34-1033 recognizes bands with apparent molecular weight of 50-65 kDa, representing multiple forms of the c-Fos protein. These bands appear in serum stimulated cells and are absent in serum-starved HeLa cells. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH: 34-1047 , used as loading control.

Product code: 34-1033

Clonality :	Polyclonal
Application :	IHC, WB
Reactivity :	Pig, Rat, Mouse, Human, Bovine

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Download TDS MSDS

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit, IgG
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	FOS
Gene ID :	2353
Uniprot ID :	P01100
Alternative Name :	Cellular oncogene fos, G0/G1 switch regulatory protein 7
Immunogen Information :	Full length recombinant human protein expressed in and purified from E. coli.

Western blots: 1:1,000-1:2,000 ICC/IF or IHC: 1:5,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus, Endoplasmic reticulum, Cytoplasm
Post transnational modification:	In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.
BioGrid:	108636. 139 interactions.