Polyclonal antibody to BFAR (BAR)
Fig:1 Western blot analysis of BAR (20-1020) in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kDa. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
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Format : | Sera |
Amount : | 50 µl |
Isotype : | Rabbit IgG |
Content : | 50 µl sera |
Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
BAR (bifunctional apoptosis regulator) is a multidomain protein that was originally identified as an inhibitor of Bax-induced apoptosis. BAR is in anchored in intracellular membranes and is thought to be a scaffold protein that may bridge components of both extrinsic and intrinsic apoptosis pathways through its antiapoptotic domains: 1. BAR contains a DED (death effector domain)-like protein interaction domain that suppresses death receptor apoptosis signaling pathways.BAR is highly expressed in the brain and expression patterns as well as functional data with neuronal cell lines suggest that BAR is involved in regulating neuronal survival. Additionally, subcellular localization studies indicate that BAR predominantly localizes to the endoplasmic reticulum (ER), irrespective of cell type. Bcl-2 family proteins also localize to the ER. There is important crosstalk between the ER and mitochondria in the execution of cell death. It is thought that both BAR and Bcl-2 proteins play a role in regulating cell death/apoptosis induced by ER stress. Dysregulation of ER homeostasis and apoptosis is thought to be involved in the pathogenesis of some human neuronal diseases, including Alzheimers, Parkinsons, polyglutamine diseases,nueronal storage diseases, prion dieases, as well as acute neurodegeration from brain trauma. Since BAR is normally widely expressed in the brain, it may have a cytoprotective function in helping neurons to survive for the entire lifetime of the organism by playing a central role in inhibiting ER initiated apoptosis.
WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Endoplasmic reticulum membrane |
Post transnational modification: | Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2. |
Tissue Specificity: | Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen. |
BioGrid: | 119435. 20 interactions. |
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