Polyclonal Antibody to Aurora kinase-A
Fig-1: Western blot analysis of Aurora kinase-A. Anti-Aurora kinase-A antibody (11-8099) was used at 1 µg/ml on (1) MCF-7,(2) SKBR3,(3) Hela,(4) 293, (5) Jurkat and (6) HepG2 lysates.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Western blot analysis: 1-2 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cytoplasm, Cytoplasm, Cytoplasm |
| Post transnational modification: | Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. |
| Tissue Specificity: | Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. |
| BioGrid: | 112666. 374 interactions. |
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