Polyclonal Antibody to ASK1 (Phospho-Ser83)

Figure 1: Western blot analysis of extracts from K562 cells using ASK1(Phospho-Ser83) Antibody 35-1169 (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

Polyclonal Antibody to ASK1 (Phospho-Ser83)

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Figure 1: Western blot analysis of extracts from K562 cells using ASK1(Phospho-Ser83) Antibody 35-1169 (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Phospho-Ser83) Antibody 35-1169 (left) or the same antibody preincubated with blocking peptide(right).

Figure 3: Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using ASK1 (Phospho-Ser83) Antibody 35-1169 .

Product code: 35-1169

Clonality :	Polyclonal
Application :	WB, IHC
Reactivity :	Human

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Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit IgG
Content :	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	MAP3K5
Gene ID :	4217
Uniprot ID :	Q99683
Alternative Name :	ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5
Immunogen Information :	Peptide sequence around phosphorylation site of serine 83 (G-S-S(p)-V-G) derived from Human ASK1.

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58. Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440. Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.

Predicted MW: 155kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Endoplasmic reticulum
Post transnational modification:	Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination leading to proteasomal degradation.
Tissue Specificity:	Abundantly expressed in heart and pancreas.
BioGrid:	110381. 99 interactions.