Polyclonal Antibody to Anti-APAF1 Antibody(Discontinued)
Figure 1: Anti-APAF1 antibody(39-2097). Western blotting: Lanes: Anti APAF1(39-2097) at 0.5ug/ml with Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: COLO320 Whole Cell Lysate at 40ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug. Predicted band size: 140 kDa. Observed band size: 140 kDa.
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| Amount : | 100 μg/vial |
| Isotype : | Rabbit IgG |
| Purification : | Immunogen affinity purified. |
| Content : | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitute : Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
| Gene : | APAF1 |
| Gene ID : | 317 |
| Uniprot ID : | O14727 |
| Alternative Name : | Apoptotic protease-activating factor 1; APAF-1; APAF1; KIAA0413 |
| Immunogen Information : | A synthetic peptide corresponding to a sequence at the N-terminal of human APAF1, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain(CARD), and an ATPase domain(NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. Additionally, APAF1 has been shown to interact with NLRP1, Caspase-9, APIP, BCL2-like 1 and HSPA4.
Western blot : 0.1-0.5μg/ml; Immunohistochemistry(Paraffin-embedded Section) : 0.5-1μg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Tissue Specificity: | Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. |
| BioGrid: | 106814. 30 interactions. |
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