Polyclonal Antibody to Adenylate Cyclase III(Discontinued)

Name: Polyclonal Antibody to Adenylate Cyclase III(Discontinued)
Brand: Abeomics
SKU: 34-1003
Price: 280.0000 USD
Availability: InStock
Rating: 4.6 (10 reviews)

Figure 1: Left: Confocal image of mixed rat neuron-glial cultures stained with our rabbit polyclonal antibody to ACIII (red) and our mouse monoclonal antibody to AlphaII-spectrin ( 34-1108 green). Note the strong, clean and specific staining of neuronal cilia. Since AlphaII-spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by the spectrin antibody. The AlphaII-spectrin antibody is also an excellent marker of neuronal plasma membranes. Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with our 34-1003 antibody (green) and our anti-Fox3/NeuN 34-1035 antibody (red), showing specific labeling of cilia next to the pyramidal neurons in CA1 hippocampus region, but not in other part of the brain. Nuclei are labeled with Dapi (blue).

Polyclonal Antibody to Adenylate Cyclase III(Discontinued)

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Figure 1: Left: Confocal image of mixed rat neuron-glial cultures stained with our rabbit polyclonal antibody to ACIII (red) and our mouse monoclonal antibody to AlphaII-spectrin ( 34-1108 green). Note the strong, clean and specific staining of neuronal cilia. Since AlphaII-spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by the spectrin antibody. The AlphaII-spectrin antibody is also an excellent marker of neuronal plasma membranes. Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with our 34-1003 antibody (green) and our anti-Fox3/NeuN 34-1035 antibody (red), showing specific labeling of cilia next to the pyramidal neurons in CA1 hippocampus region, but not in other part of the brain. Nuclei are labeled with Dapi (blue).

Figure 2: Western blots of rat olfactory epithelium (OE) and frontal cortex (FC). Our antibody stains bands at about 200 kDa in olfactory epithelium, a tissue which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is also less heavily glycosylated, so that a much less prominent band is seen at about 160 kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.

Product code: 34-1003

Clonality :	Polyclonal
Application :	IHC, WB
Reactivity :	Pig, Rat, Mouse, Human, Bovine

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit, IgG
Content :	Supplied as an aliquot of affinity purified at 1 mg/ml.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	Adcy3
Gene ID :	64508
Uniprot ID :	P21932
Alternative Name :	ATP pyrophosphate-lyase 3, AC-III, Adenylate cyclase type III, Adenylate cyclase, olfactive type, AC3, Adenylyl cyclase 3
Immunogen Information :	C-terminal peptide of rat ACIII, PAAFPNGSSVTLPHQVVDNP with a Cys added to the N-terminus to allow coupling to KLH.

Western blots: 1:5,000-1:10,000. ICC/IF or IHC: 1:500-1:1,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

1. Bowie E, Goetz SC. TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons. Elife. 2020 Jan 14;9. pii: e51166. doi: 10.7554/eLife.51166. [Epub ahead of print] PubMed PMID: 31934864.

Subcellular location:	Cell membrane, Golgi apparatus, Cell projection, Cytoplasm
Post transnational modification:	Rapidly phosphorylated after stimulation by odorants or forskolin. Phosphorylation by CaMK2 at Ser-1076 down-regulates enzyme activity.
Tissue Specificity:	Detected on cilia on the olfactory epithelium (at protein level) (PubMed:2255909, PubMed:25908845). Detected on cilia on the olfactory epithelium.
BioGrid:	249093. 1 interactions.