PE conjugated Anti-Human IL-17A Mab (Clone: 9F9)
Figure 1: Flow cytometry intracellular staining pattern of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Roll over image to zoom in
Shipping Info:
For estimated delivery dates, please contact us at [email protected]
Amount : | 100 tests |
Isotype : | Mouse IgG1 kappa |
Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Content : | Storage Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Specificity : The mouse monoclonal antibody 9F9 recognizes human interleukin 17A (IL-17A; secreted or intracellular).
Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
There are currently no product reviews
|